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MapPairs® Human Markers

Thousands of unlabeled human markers are available. The collection includes CEPH markers developed at Genoscope, Centre National de Séquençage (1) as well as others from a variety of groups, including the Marshfield Institute and the Cooperative Human Linkage Center (2). Fluorescent-labeled MapPairs® human markers are also available.* The labeled markers can be multiplexed based on both allele size and dye color, allowing multiple genotypes per lane. Hundreds of M13-tailed human markers are also available. A complete list of MapPairs® Human Markers is available here.

Product   Concentration Quantity Cat. no.  
MapPairs® Human Markers
Unlabeled 20.0 µM 200 µl 20501
Fluorescent-labeled 8.0 µM 200 µl 20508RG
with M13 tails 20.0 µM 200 µl 20510RG
* Fluorescent labels available are: D2, D3, D4, FAM™, HEX™, TET™; and Cy™5.

References:

  1. Gyapay, G. et al. (1994) Nature Genetics 7: 246-339.
  2. Dubovsky, J. et al. (1995) Human Mol. Genetics 4 (3): 449-452.

Genome-Wide Human Screening Sets

Developed at the Marshfield Institute, these genome-wide sets consist of a larger scan of 405 markers (Version 10) and a smaller scan of 200 markers (Version 10a) approximately 9 cM and 18 cM apart, respectively. They are available unlabeled or labeled with fluorescent dyes. Markers in the labeled sets are multiplexed based on both allele size and dye color, allowing multiple genotypes per lane. As a result, Version 10 can be run in 36 lanes, and Version 10a can be run in 17 lanes. The Single Chromosome Scan Set was developed at the Magainin Institute of Molecular Medicine (1). The set consists of 516 tri- and tetra-nucleotide repeats approximately 7 cM apart and is labeled with dyes.* Multiplexed by chromosome, allele size, and dye color, the set can be run in 43 lanes. A complete list of MapPairs® Markers in the Genome-Wide Human Screening Set is available here.

Product   Concentration Quantity Cat. no.  
 
Version 10 - Unlabeled 20.0 µM 200 µl 20505.1
Version 10 - Fluorescent-labeled 8.0 µM 200 µl 20505.2
Version 10a - Unlabeled   20.0 µM 200 µl 20505.5
Version 10a - Fluorescent-labeled 8.0 µM 200 µl   20505.6
Low Volume Version 10 8.0 µM 50 µl 20505.9
Low Volume Version 10a   8.0 µM 50 µl 20505.10
Single Chromosome Scan Set 8.0 µM 200 µl 20505.11
* The fluorescent dyes available are FAM™, HEX™, and TET™.

Reference:

  1. Levitt, R. et al. (1998) Am. J. Human Genetics 63: A256.

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