MapPairsdate® Microsatellite Markers
Overview of Ideal Genetic Markers for Linkage Analysis
MapPairs® microsatellite markers are PCR primers that flank a simple sequence repeat (SSR). SSRs occur in most
higher eukaryotes and can serve as markers for genetic analysis and positional cloning (1-4). The length of polymorphisms
associated with SSRs have been utilized in model organisms to localize multiple quantitative trait loci (QTLs) ascribed to
complex polygenic diseases (5-7). A variety of labeling methods are available. A single pair of MapPairs® markers
includes flanking primers, suspended in solution and ready-to-use. Annealing temperatures, denaturation temperatures,
and Mg2+ concentration may need to be optimized for best results.
References:
-
Weber, J.L. et al.
(1989) Amer. J Hum. Genet. 44: 388.
-
Risch, N. et al. (1995)
Nature Genetics 9: 152-158.
-
Zhang, Y. et al. (1995)
Nature 372: 425-431.
-
Hastbacka, J. et al.
(1994) Cell 78: 1073-1087.
-
Davies, J.L. et al.
(1994) Nature 371: 130-136.
-
Hashimoto et al. (1994)
Nature 371: 161-164.
-
Jacob, H.J. et al.
(1993) Nature Genetics 2: 56-60.
| 
