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Order BAC/PAC clones and BAC/PAC plates
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California Institute of Technology
In collaboration with Hiroaki Shizuya at the California Institute of Technology, genomic BAC libraries of
the human and mouse were constructed. The sources of human DNA are the 978SK cell line and human sperm.
Four distinct human BAC libraries have been created by the California Institute of Technology. The B & C libraries
together represent a 15X coverage of the human genome. It is from these libraries that DNA pools for PCR screening have been made. Users of
the DNA pools will be able to screen down to a specific plate address.
Yu-Ling Sheng and Sangdun Choi developed the human BAC D library in Shizuya's lab at the California Institute of Technology.
The library is offered as individual clones. The 'D' library provides a 17X coverage of the human genome and meets the NIH guidelines for the
protection of the anonymity of the donors. DNA pools for PCR screening are not available for the 'D' library.
Individual clones from the human BAC libraries are available. Clone addresses may have been determined by screening efforts
or through literature. Clones are provided as a glycerol stocks. Please specify clone address when ordering.
Ref: 1. Shizuya, H., B. Birren, U-J. Kim, V. Mancino, T. Slepak, Y. Tachiiri, M. Simon (1992) Proc. Natl. Acad. Sci., USA 89: 8794-8797.
2. Kim, U-J., B. Birren, T. Slepak, V. Mancino, C. Boysen, H.L. Kang, M.I. Simon, H. Shizuya (1996) Genomics 34 213-218.
3. Swiatek, P.J. and T. Gridley (1993) Genes and Development 7: 2071-2084.
Facts about Mouse and Human BACs from CalTech
Vector pBeloBAC11
Host HS996 (modified DH10B)
Avg Insert Size 130 kb
CTB and CTC Human BAC DNA Pools (B &
C Libraries)
Human BAC DNA Pools are suitable for screening by
PCR; a two level screening process at the end of which you have the
plate location of your clone. The pools consist of 96 superpools and
the corresponding 768 plate pools. Only 104 PCR reactions are required
to obtain the plate location of a clone. Row and Column pools are no
longer available. Sufficient DNA is provided for 50 PCR screenings.
Mouse BAC DNA Pools
Mouse BAC DNA Pools are suitable for screening by
PCR; a two level screening process at the end of which you have the
plate location of your clone. The pools consist of 72 superpools and
the corresponding 576 plate pools. Only 80 PCR reactions are required
to obtain the plate location of a clone. Sufficient DNA is provided
for 50 PCR screenings.
Mouse BAC Clones
Individual clones from the mouse BAC library. Clone
addresses may have been determined by screening efforts or through literature.
Clones are provided as glycerol stocks. Please specify clone address
when ordering.
Roswell Park Cancer Institute
RPCI-11
The RPCI-11 Human BAC library was constructed in Pieter deJong's laboratory at the Roswell Park Cancer Institute
by Kazutoyo Osoegawa and Minako Tateno. Male white blood cells were used as the DNA source. Partially EcoRI digested DNA was cloned in to
the pBACe3.6 vector. A picture and the sequence of this vector can be viewed at http://bacpac.chori.org/
The library represents approximately a 25X coverage of the human genome. Individual clones are available from the RPCI-11 Human BAC library. Clones
are provided as glycerol stocks. Please specify clone address when ordering. Clone address example: 341L6 (plate 341 well L6)
RPCI-22
The RPCI-22 Mouse BAC library was constructed in Pieter deJong's laboratory at the Roswell Park Cancer Institute
by Kazutoyo Osoegawa. This library is offered as individual clones. It contains more than 211,000 clones providing 10.9X coverage of
the mouse genome. Stability of these clones is high and chimerism is low to nonexistent. Detailed information about the vector used
and the construction of the library can be viewed at: http://bacpac.chori.org/.
Please specify clone address when ordering
Average Insert Size: 154 kb
DNA Source: female mouse spleen genomic DNA (129S6/SvEvTac)
Vector: pBACe3.6
Host Strain: DH10B
Genomic Coverage: 10.9X
RPCI-22 Mouse BAC Clones
Individual clones from the RPCI-22 mouse BAC library.
Clones are provided as glycerol stocks. Please specify clone address
when ordering.
RPCI-23
The RPCI-23 Mouse BAC library was constructed in Pieter deJong's laboratory at the Roswell Park Cancer Institute
by Kazutoyo Osoegawa and Minako Tateno. This library is offered as individual clones. It contains more than 170,000 clones
providing 11.2X coverage of the mouse genome. The DNA source of this library is female mouse kidney/brain (C57BL/6J). Stability of
these clones is high and chimerism is low to nonexistent. Detailed information about the vector used and the construction of the library
can be viewed at: http://bacpac.chori.org/. Please specify clone address when ordering.
Average Insert Size: 197 kb
DNA Source: female mouse kidney/brain genomic DNA (C57BL/6J)
Vector: pBACe3.6
Host Strain: DH10B
Genomic Coverage: 11.2X
RPCI-23 Mouse BAC Clones
Individual clones from the RPCI-23 mouse BAC library.
Clones are provided as a bacterial culture. Please specify clone address
when ordering.
Additional BAC/PAC Resources
AB1380
The yeast host strain used in the construction of
some of the YAC libraries.
HS996
E. coli host strain used in constructing the BACs.
A modification of DH10B/r.
978SK DNA
The human cell line used as insert in the construction
of a portion of the human BAC library.
pBeloBAC11
The plasmid vector used in the construction of the
mouse and human BAC libraries.
BAC End Primers
These primers flank the HindIII cloning site of pBeloBAC11 and can be used for BAC end sequencing.
Forward 5' GAT-TAC-GCC-AAG-CTA-TTT-AGG-TGA-CAC-TAT-AGA-ATA-C-3'
Reverse 5' TAA-TAC-GAC-TCA-CTA-TAG-GGC-GAA-TTC-GAG-CTC-GG-3'
Ref: Wang, et al (1994) Genomics 24 527-534.
Shizuya, H., et.al. (1992) Proc. Natl. Acad. Sci. USA 89, 8794-8797.
BAC End Primers Short
Short version of catalog number 96060.
Forward 5' CAA-GCT-ATT-TAG-GTG-ACA-CTA-TAG-3'
Reverse 5'TAA-TAC-GAC-TCA-CTA-TAG-GGC-GA-3'
PAC Genomic Libraries
Roswell Park Cancer Institute
Developed in Peiter deJong's laboratory at the Roswell
Park Cancer Institute, the RPCI-4, 5, and 6 libraries contain more than
335,000 clones providing a 14X coverage of the human genome.
Ref: Iannou, P., Amemiya, C., Garnes, J., Kroisel,
P., Shizuya, H., Chen, C., Baster, M., deJong, P. (1994) Nature Genetics, 6 84-89.
The Human PAC Library is divided into the
following 5 sub-libraries:
- RPCI.1 - Plate Numbers 1-321
- RPCI.3 - Plate Numbers 322-528
- RPCI.4 - Plate Numbers 529-816
- RPCI.5 - Plate Numbers 817-1200
- RPCI.6 - Plate Numbers 1-240
Due to the fact that sub-libraries 1 and 6 overlap,
please note that if your clone plate number falls between 1 and 240,
we will need to know which sub-library your clone is from.
RPCI-1, 3, 4, 5, 6 Human PAC Clones
Individual clones from the RPCI1, 3, 4, 5 and 6
libraries described above are available as glycerol stocks. Clone addresses
may have been determined by screening efforts or through literature.
Please specify clone address and library when ordering.
RPCI Mouse PAC Resources
The RPCI-21 mouse PAC library was constructed in Pieter deJong's laboratory at the Rosewell Park Cancer Institute
by Kazutoyo Osoegawa. Female 129/SvevTACfBr mouse spleen genomic DNA (partially MboI digested) was cloned into the BAMHI sites of the
pPAC4 vector. The library has been arrayed into 672 microtiter plates (384-well). The average insert size of the clones is 146kb, representing
a 12X coverage of the mouse genome. Characterization of this library is ongoing and can be viewed on the Roswell Park web page at
http://bacpac.chori.org/.
RPCI-21 Mouse PAC Clones
Individual clones from the RPCI-21 mouse PAC library.
Clones are provided as glycerol stocks. Please specify clone address
when ordering.
RPCI-31 Rat PAC Resources
The RPCI31 Rat PAC library was constructed in tha
lab of Pieter deJong at the Roswell Park Cancer Institute by Peng Yeong
Woon. Female Brown Norway (BN/ssNHsd) rat spleen genomic DNA (partially
MboI digested) was cloned into the BamHI sites of the pPAC4 vector.
The library has been arrayed into 614 microtiter plates (384 well).
The average insert size of the clones is 147 kb representing approximately
10-fold coverage of the rat genome. Characterization of this library
is ongoing and can be viewed on the Roswell Park Cancer Institute web
page http://bacpac.chori.org/
RPCI31 Rat PAC Clones
Individual clones from the RPCI31 rat PAC library.
Please specify clone address when ordering.
BAC and PAC links to more information
NCBI
Clone Registry
NIH
(NHGRI) BAC Resource Network
NSF
BAC awards
Pieter
de Jong's BAC/PAC genomics resource center (CHORI)
Caltech
Genome Research Laboratory
Julie
Korenberg's YAC/BAC/PAC page, including human BAC-PAC map
DOE
BAC website
Texas
A&M University BAC Center
Clemson
University Genomics Institute
Arizona
Genomics Institute
Jean
Michel Vos' laboratory
BAC/PAC
end sequencing strategy for the human genome
ET
homologous recombination cloning page (EMBL)
Contact Info
U.S. Academic and Industrial Orders
To Order: (800) 955- 6288, Option 1
Fax: (800) 331-2286
Hours of operation: 8:00 AM - 8:00 PM EST
U.S. Government Orders
To Order: (888) 584-8929
Fax: (888) 584-8930
Hours of operation: 8:00 AM - 8:00 PM EST
U.S. TECH-LINE
To Order: (800) 955-6288
Fax: (800) 352-1468
FAQ/ASK US
Hours of operation: 9:00 AM - 8:00 PM EST
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